Generation of hypoxanthine phosphoribosyltransferase gene knockout rabbits by      homologous recombination and gene trapping through somatic cell nuclear transfer.

Yin M; Jiang W; Fang Z; Kong P; Xing F; Li  Y; Chen X; Li S

doi:10.1038/srep16023

Generation of hypoxanthine phosphoribosyltransferase gene knockout rabbits by      homologous recombination and gene trapping through somatic cell nuclear transfer.

Yin M; Jiang W; Fang Z; Kong P; Xing F; Li Y; Chen X; Li S

Scientific Reports, 2015, 5: 16023-16023.

DOI: 10.1038/srep16023

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Summary

The rabbit is a common animal model that has been employed in studies on various       human disorders, and the generation of genetically modified rabbit lines is      highly desirable. Female rabbits have been successfully cloned from cumulus      cells, and the somatic cell nuclear transfer (SCNT) technology is well      established. The present study generated hypoxanthine phosphoribosyltransferase      (HPRT) gene knockout rabbits using recombinant adeno-associated virus-mediated      homologous recombination and SCNT. Gene trap strategies were employed to enhance       the gene targeting rates. The male and female gene knockout fibroblast cell lines      were derived by different strategies. When male HPRT knockout cells were used for      SCNT, no live rabbits were obtained. However, when female HPRT(+/-) cells were      used for SCNT, live, healthy rabbits were generated. The cloned HPRT(+/-) rabbits      were fertile at maturity. We demonstrate a new technique to produce gene-targeted      rabbits. This approach may also be used in the genetic manipulation of different       genes or in other species.

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