Output ID:  1000017237635
Category:  期刊论文
Journal Article Title:  :  Dual-primer self-generation SERS signal amplification assay for PDGF-BB using label-free aptamer
Abstract:  Highly sensitive detection of proteins, especially those associated with cancers, is essential to biomedical research as well as clinical diagnosis. In this work, a simple and novel one-two-three signal amplification surface-enhanced Raman scattering (SERS) method for the detection of protein is fabricated by using label-free aptamer and dual-primer self-generation. Platelet-derived growth factor B-chain (PDGF-BB) is selected as the model protein. The one-two-three cascade DNA amplification means one target-aptamer binding event, two hairpin DNA switches and three DNA amplification reactions. This strategy possesses some remarkable features compared to conventional signal amplification methods: (i) A smart probe including a label-free aptamer is fabricated, for suitable hybridization without hindering the affinity of the aptamer toward its target. (ii) Using the unique structure switch of the aptamer and cooperator, a one-two-three working mode is developed to amplify the SERS signal...
Keywords:  Surface-enhanced Raman scattering; PDGF-BB; Signal amplification 
Authors:  Ye, SuJuan; Zhai, XiaoMo; Wu, YanYing; *Kuang, ShaoPing
Journal Name:  Biosensors and Bioelectronics 
Status:  Published
Publication Date/Period:  2016-5-15 
Volume No.:  79 
Pages:  130-135 
Indexed by:  scie
DOI:  10.1016/j.bios.2015.11.090
Times Cited:  1
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Last update date:  2019/02/18 12:56:14