Isolation of Mhc class I cDNAs from the axolotl Ambystoma mexicanum

作者：Sammut B; Laurens V; Tournefier A

来源：Immunogenetics, 1997, 45(5): 285-294.

DOI：10.1007/s002510050207

Class I major histocompatibility complex (Mhc) cDNA clones were isolated from axolotl mRNA by polymerase chain reaction (PCR) and by screening a cDNA phage library. The nucleotide and predicted amino acid sequences show definite similarities to the Mhc class Ia molecules of higher vertebrates. Most of the amino acids in the peptide binding region that dock peptides at their N and C termini in mammals are conserved. Several amino acids considered to be important for the interaction of beta(2)-microglobulin with the Mhc alpha chain are also conserved in the axolotl sequence. The fact that axolotl class IA cDNAs are ubiquitously expressed and highly polymorphic in the al and alpha 2 domains suggests the classical nature of axolotl class IA genes.

• 出版日期1997