Nucleostemin expression in squamous cell carcinoma of the head and neck

作者：Cada Zdenek; Boucek Jan; Dvorankova Barbora; Chovanec Martin; Plzak Jan; Kodet Roman; Betka Jan; Pinot Gian L; Gabius Hans Joachim; Smetana Karel Jr^*

来源：Anticancer Research, 2007, 27(5A): 3279-3284.

• basal cell carcinoma
• beta-catenin
• epidermis
• keratin
• lectin
• nucleolus
• nucleostemin

Background: This study presents initial data on presence of nucleostemin - a nucleolar protein typical of stem cells in the normal squamous epithelium of the oropharynx and larynx - in squamous cell carcinoma originating from these epithelia. Materials and Methods: Differentiation and proliferation markers such as keratins, P-catenin, galectin-1, and Ki-67 were studied in parallel with nucleostemin for defining cell characteristics. Results: Nucleostemin was detected in nucleoli of both proliferating basal cells and terminally differentiated suprabasal cells of normal epithelium and in tumor cells. Importantly, malignant transformation was connected with a significant enlargement of nucleosteminpositive nucleoli in these cell types. Conclusion: Detection of nucleostemin in head and neck cancer cells, together with the size of nucleoli, may be important in the evaluation of tumor difterentiation and biology.

• 出版日期2007-10