Down-Regulation of TRPM5s During the Development of the Rat Neocortex and Hippocampus

作者：Sun, Feng Peng^*; Gao, Tian Ming

来源：Neurophysiology, 2013, 45(2): 112-119.

DOI：10.1007/s11062-013-9345-x

• transient receptor potential channels (TRPMs)
• TRPM5s
• development
• neocortex
• hippocampus
• transcripts
• PCR

The transient receptor potential (TRP) superfamily of cation membrane channels includes a large number of recently identified protein molecules that share the sequence homology with the Drosophila protein named after a phototransduction mutant of this insect called trp. Believing that one or more TRP channels might be involved in the process of neural development, we examined the possibility by searching the full profile of TRPC1-7, TRPV1-6, TRPM1-8, and TRPA1 channels during the development of the neocortex and hippocampus of rats (from the embryonic state to adults). The RT-PCR experiment was performed with mRNA isolated from the above-mentioned cerebral structures. Developmental changes were identified in transcripts for the profiles of TRPC1-7, TRPV1-6, TRPM1-8, and TRPA1. Down-regulation of exclusively TRPM5, whose mRNA expression level gradually decreased in the course of pre- and post-natal development of both neocortex and hippocampus, was the most striking finding. Our results suggest that TRPM5s might have some important function in neural development. There is a possibility that TRPM5s are key components of the physiological function for sensing the environmental temperature in the developmental period of rats.

• 出版日期2013-3
• 单位南方医科大学