A naphthalimide-rhodamine two-photon fluorescent turn-on probe for hypochlorous acid by desulfurization-cyclization and fluorescence resonance energy transfer

作者：Yao, Shan-kun; Qian, Ying^*

来源：Sensors and Actuators B: Chemical , 2017, 252: 877-885.

DOI：10.1016/j.snb.2017.06.091

• Fluorescence resonance energy transfer
• Hypochlorous acid sensor
• Desulfurization-cyclization reaction
• Naphthalimide-rhodamine
• Two-photon excited fluorescence

A new ratiometric naphthalimide-rhodamine two-photon fluorescent turn-on probe RHSDN with a 'naked-eye' recognition capability for hypochlorous acid has been rationally designed, in which rhodamine-thiosemicarbazide and pyridine-naphthalimide were connected by phenyl spacer. In presence of HClO, RHSDN exhibits high sensitivity, selectivity toward HClO at trace levels over other anions/ROS based on hypochlorous acid-triggered intramolecular desulfurization-cyclization of rhodamine-thiosemicarbazide. Under the Ti: sapphire pulsed laser's 800 nm irradiation, rhodamine spirolactam closed-ring of RHSDN toward hypochlorous acid affords an OFF-ON signal response with an appearance and drastic enhancement of fluorescence emission peak at 600 nm accompanied by a dramatic color change from colorless to brightly pink after treatment with hypochlorous acid. Besides, the chemodosimeter RHSDN for the detection of HClO with fast response time, high sensitivity with low detection limit of 9.6 x 10(-8) M and works excellently within a wide pH range of 4-11, addressing the existing time and pH dependency issue. Moreover, RHSDN has been employed for the imaging of artificially loaded HClO with satisfying results, which further displayed its valuable applications in biological systems.

• 出版日期2017-11
• 单位东南大学