A RAPID UPLC-MS/MS METHOD FOR SIMULTANEOUS DETERMINATION OF FIVE ANTHRAQUINONES IN RAT PLASMA: APPLICATION TO A PHARMACOKINETIC STUDY

作者：Zhang, Lin; Chang, Jin-Hua; Liu, Li-Yan; Fu, Qiang; Liu, Cui-Zhe^*

来源：Acta Medica Mediterranea, 2018, 34(1): 175-180.

DOI：10.19193/0393-6384_2018_1_29

• Rhubarb anthraquinones
• rat plasma
• pharmacokinetics
• UPLC-MS/MS

Introduction: A rapid and simple UPLC-MS/MS method was established and validated for simultaneous determination of aloe-emodin, rhein, emodin, chrysophanol and physcion in rat plasma.
Materials and methods: After sample preparation by a simple protein precipitation, the mass detection was conducted in multiple reaction monitoring using an API 6500 triple quadruple in negative-ion mode with an electrospray ionization interface. Gradient elution was performed with a mobile phase composed of acetonitrile and water containing 0.1% formic acid at a flow rate of 0.6 mL/min.
Results: The retention time of emodin, aloe-emodin, chrysophanol, physcion, rhein and IS was at 1.54, 1.04, 1.67, 1.69, 1.09 and 1.59 min, respectively.
Conclusion: This method exhibited acceptable precision, accuracy, recoveries, matrix effect and carry-over effect, which was superior to previous methods in analysis time and sample throughput.

• 出版日期2018
• 单位承德医学院; 沈阳药科大学