A sample preparation protocol for H-1 nuclear magnetic resonance studies of water-soluble metabolites in blood and urine

作者：Sheedy John R^*; Ebeling Peter R; Gooley Paul R; McConville Malcolm J

来源：Analytical Biochemistry, 2010, 398(2): 263-265.

DOI：10.1016/j.ab.2009.11.027

• NMR
• Metabolites
• Biofluids

We describe a general protocol for preparing protein-containing biofluids for H-1 nuclear magnetic resonance (NMR) metabolomic studies. In this protocol, untreated samples are diluted in deuterated solvents to precipitate proteins and recover metabolites quantitated relative to standard reference compounds such as 3-trimethylsilylpropionic acid (TSP) and 2,2-dimethyl-2-silapentane-5-sulfonic acid (DSS). The efficacy of this protocol was tested using a bovine serum albumin/metabolite mix and human serum samples. This sample preparation method can be readily applied to any protein-containing biofluid for H-1 NMR studies.

• 出版日期2010-3-15