科研之友
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摘要
The thermodynamic stability of etanercept in different concentrations of the protective agent was investigated. The approaches of umbrella sampling and steered molecular dynamic simulation were adopted to study the dissociation of dimer etanercept with Gromacs software and amber99sb-ildn united atomic force field. The results indicated that the trehalose has ability to enhance the stability of dimer etanercept, and the protective effect of etanercept with trehalose and mannitol is better than with pure trehalose. In addition, the type of protective agent and alternative specific adsorption position can impact the effect of protecting active structure of etanercept observably.
- 出版日期2016-2-10
- 单位上海理工大学
