Differentiation of Human Umbilical Cord Wharton's Jelly-Derived Mesenchymal Stem Cells Into Germ-Like Cells In Vitro

作者：Huang Peng; Lin Li Min; Wu Xiao Ying; Tang Qiu Ling; Feng Xue Yong; Lin Guang Yu^*; Lin Xiaobo; Wang Hong Wu; Huang Tian Hua; Ma Lian

来源：Journal of Cellular Biochemistry, 2010, 109(4): 747-754.

DOI：10.1002/jcb.22453

• HUMAN UMBILICAL CORD
• MESENCHYMAL STEM CELL
• GERM LIKE CELLS
• IN VITRO DIFFERENTIATION

Recent studies have demonstrated that mesenchymal stem cells could differentiate into germ cells under appropriate conditions. We sought to determine whether human umbilical cord Wharton's jelly-derived mesenchymal stem cells (HUMSCs) could form germ cells in vitro. HUMSCs were induced to differentiate into germ cells in all-trans retinoic acid, testosterone and testicular-cell-conditioned medium prepared from newborn male mouse testes. HUMSCs formed "tadpole-like" cells after induction with different reagents and showed both mRNA and protein expression of germ-cell-specific markers Oct4 (POUE5), Ckit, CD49(f) (alpha 6), Stella (DDPA3), and Vasa (DDX4). Our results may provide a new route for reproductive therapy involving HUMSCs and a novel in vitro model to investigate the molecular mechanisms that regulate the development of the mammalian germ lineage. J. Cell. Biochem. 109: 747-754, 2010.

• 出版日期2010-3-1
• 单位汕头大学