Preparative separation of gambogic acid and its C-2 epimer using recycling high-speed counter-current chromatography

作者：Bin Han Quan; Song Jing Zheng; Qiao Chun Feng; Wong Lina; Xu Hong Xi^*

来源：Journal of Chromatography A, 2006, 1127(1-2): 298-301.

DOI：10.1016/j.chroma.2006.07.044

• recycling counter-current chromatography
• preparative separation
• Garcinia hanburyi
• epimer
• gambogic acid
• epigambogic acid

A recycling counter-current chromatographic system was first set up with a high-speed counter-current chromatography instrument coupled with a column switching valve. This system was first successfully applied to the preparative separation of epimers, gambogic acid and epigambogic acid from Garcinia hanburyi using n-hexane-methanol-water (5:4: 1, v/v/v) as the two-phase solvent system. As a result, 28.2 mg gambogic acid and 18.4 mg epigambogic acid were separated from 50 mg of mixture. Their purities were both above 97% as determined by HPLC. The chemical structures were then identified by their H-1 NMR and C-13 NMR spectra.

• 出版日期2006-9-15