AN ALTERNATE NONISOTOPIC TECHNIQUE OF PCR-MEDIATED ALLELE-SPECIFIC OLIGONUCLEOTIDE ANALYSIS FOR THE DETECTION OF A POINT MUTATION

作者：NAKAJIMA H^*; HAMAGUCHI T; TOMITA K; UENAKA R; YAMAGATA K; HANAFUSA T; KUWAJIMA M; NOGUCHI T; TANAKA T; KONO N; MATSUZAWA Y

来源：Research Communications in Chemical Pathology and Pharmacology, 1993, 79(1): 3-10.

We report a simple protocol for the allele analysis of a point mutation using polymerase chain reaction. Conditions have been determined to use probes prepared for the conventional allele-specific oligonucleotide hybridization method directly. After amplification of the genomic region containing the mutation site, the nested polymerase chain reaction was performed using an allele-specific oligonucleotide probe as one side of the primer. Specificity of the amplification was achieved by decreasing the primer concentration in a two segment thermal cycling pattern. This protocol does not require additional special primers and an allelic mutation can be determined non-isotopically.

• 出版日期1993-1