Additive Regulation of Adiponectin Expression by the Mediterranean Diet Olive Oil Components Oleic Acid and Hydroxytyrosol in Human Adipocytes

作者：Scoditti Egeria; Massaro Marika; Carluccio Maria Annunziata; Pellegrino Mariangela; Wabitsch Martin; Calabriso Nadia; Storelli Carlo; De Caterina Raffaele^*

来源：PLos One, 2015, 10(6): e0128218.

DOI：10.1371/journal.pone.0128218

Adiponectin, an adipocyte-derived insulin-sensitizing and anti-inflammatory hormone, is suppressed in obesity through mechanisms involving chronic inflammation and oxidative stress. Olive oil consumption is associated with beneficial cardiometabolic actions, with possible contributions from the antioxidant phenol hydroxytyrosol (HT) and the monounsaturated fatty acid oleic acid (OA, 18:1n-9 cis), both possessing anti-inflammatory and vasculo-protective properties. We determined the effects of HT and OA, alone and in combination, on adiponectin expression in human and murine adipocytes under pro-inflammatory conditions induced by the cytokine tumor necrosis factor(TNF)-alpha. We used human Simpson-Golabi-Behmel syndrome (SGBS) adipocytes and murine 3T3-L1 adipocytes as cell model systems, and pretreated them with 1-100 mu mol/L OA, 0.1-20 mu mol/L HT or OA plus HT combination before stimulation with 10 ng/mL TNF-alpha. OA or HT significantly (P<0.05) prevented TNF-alpha-induced suppression of total adiponectin secretion (by 42% compared with TNF-alpha alone) as well as mRNA levels (by 30% compared with TNF-alpha alone). HT and OA also prevented-by 35%-TNF-alpha-induced downregulation of peroxisome proliferator-activated receptor PPAR gamma. Co-treatment with HT and OA restored adiponectin and PPAR gamma expression in an additive manner compared with single treatments. Exploring the activation of JNK, which is crucial for both adiponectin and PPAR gamma suppression by TNF-alpha, we found that HT and OA additively attenuated TNF-alpha-stimulated JNK phosphorylation (up to 55% inhibition). In conclusion, the virgin olive oil components OA and HT, at nutritionally relevant concentrations, have additive effects in preventing adiponectin downregulation in inflamed adipocytes through an attenuation of JNK-mediated PPAR gamma suppression.

• 出版日期2015-6-1