A new xylanase from Streptomyces megasporus DSM 41476 with high yield of xylobiose

作者：Shi, Pengjun; Qiu, Zhenhua; Bai, Yingguo; Yuan, Tiezheng; Huang, Huoqing; Pan, Xia; Yang, Peilong; Zhang, Wei; Yao, Bin^*

来源：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2012, 28(2): 687-692.

DOI：10.1007/s11274-011-0863-7

• Streptomyces megasporus
• Xylanase
• Pichia pastoris
• Xylobiose

A new xylanase gene, xynBM4, was cloned from Streptomyces megasporus DSM 41476 and expressed in Pichia pastoris. The full-length gene consists of 1,443 bp and encodes 480 amino acids including a putative 49-residue signal peptide. The deduced amino acid sequence of xynBM4 shows the highest identity of 66.3% to the xylanase Xys1L from Streptomyces halstedii JM8. The purified recombinant XYNBM4 had a high specific activity of 350.7 U mg(-1) towards soluble wheat arabinoxylan, exhibited optimal activity at pH 6.0 and 57A degrees C, showed broad pH adaptability (> 75% of the maximum activity at pH 2.5-9.0), was resistant to neutral proteases and most chemicals, and produced simple products. The hydrolysis products of birchwood xylan and corncob xylan were predominantly xylobiose (76.9 and 90.8%, respectively) and no xylose. These characteristics suggest that XYNBM4 has potential in various applications, especially in the food industry.

• 出版日期2012-2
• 单位中国农业科学院