An accurate, specific, sensitive, high-throughput method based on a microsphere immunoassay for multiplex detection of three viruses and bacterial fruit blotch bacterium in cucurbits

作者：Charlermroj Ratthaphol; Makornwattana Manlika; Himananto Orawan; Seepiban Channarong; Phuengwas Sudtida; Warin Nuchnard; Gajanandana Oraprapai; Karoonuthaisiri Nitsara

来源：Journal of Virological Methods, 2017, 247: 6-14.

DOI：10.1016/j.jviromet.2017.05.006

• Microsphere immunoassay
• Plant pathogens
• Multiplex detection
• Validation
• Cucurbits

Abstract(#br)To employ a microsphere immunoassay (MIA) to simultaneously detect multiple plant pathogens (potyviruses, Watermelon silver mottle virus , Melon yellow spot virus, and Acidovorax avenae subsp. citrulli ) in actual plant samples, several factors need to be optimized and rigorously valida...

• 出版日期2017-9