Application of Repetitive Extragenic Palindromic Elements Based on PCR in Detection of Genetic Relationship of Lactic Acid Bacteria Species Isolated from Traditional Fermented Food Products

作者：Tafvizi F^*; Ebrahimi M Tajabadi

来源：Journal of Agricultural Science and Technology, 2015, 17(1): 87-98.

• 16S rDNA sequencing
• REP-PCR fingerprinting

Tarkhineh is one of the traditional foods in Iran and a rich source of probiotic bacteria. The objective of this study was to identify the probiotic bacteria isolated from Tarkhineh using 16S rRNA gene sequencing and molecular typing with repetitive extragenic palindromic-PCR (REP-PCR). In total, 20 different bacteria were isolated from traditional dairy products and Tarkhineh. Molecular identification of the isolates was carried out by 16S rRNA gene sequencing, and DNA sequences of isolates deposited in GenBank. The REP-PCR reaction by REP1R-I, REP2-I and REP1R-I+REP2-I markers was performed for fingerprinting and characterization of the isolates. Unweighted pair group method with arithmetic mean (UPGMA) clustering methods were performed based on Dice similarity. The REP1R-I primer grouped isolates into three, and REP2-I and REP1R-I+REP2-I grouped all isolates into four main clusters in dendrograms. In all analyses, isolates of Lactobacillus casei, Lactobacillus brevis, Lactobacillus plantarum, and Entrococcus facium formed separate clusters. The results of sequencing corresponded to clustering in the dendrogram. According to the results, REPPCR is an accurate technique for determining the genetic diversity of lactic acid bacteria species.

• 出版日期2015-2