Antibody-mediated binding of fluorescent HIV Gag and influenza nucleoprotein tetramers to blood cells

作者：Reiche Sven; Bussmann Bianca M; Dwai Yamen; Jassoy Christian^*

来源：Immunobiology, 2010, 215(3): 223-229.

DOI：10.1016/j.imbio.2009.02.004

• Flow cytometry
• B-lymphocyte
• Fluorescent tetramer
• Complement receptor
• Fc gamma receptor
• HIV
• Influenza

Antibody-mediated elimination of foreign antigens contributes to immune protection from viral infection. We have generated phycoerythrin-conjugated HIV-1 Gag/p24 and influenza virus nucleoprotein tetramers for flow cytometric analysis of blood cell populations involved in antibody-mediated immunity. We show that in the presence of antigen-specific antibodies fluorescent antigen tetramers bound to different blood cell populations including granulocytes, monocytes and lymphocytes. Binding to B-lymphocytes was particularly efficient. The interaction was primarily mediated by complement. Fey receptor-mediated antigen binding by B-cells was significantly less effective. The study shows that fluorescent antigen tetramers are useful to quantify cell populations involved in complement- and Fc gamma-mediated immune responses in viral infections.

• 出版日期2010-3