Antioxidant activities and HepG2 cells growth inhibitory capacity of whole plant ethanol extracts (Eclipta alba Hassk and Mesona procumbens Hemsl)

作者：Le, Quang-Ung; Lay, Horng-Liang^*; Wu, Ming-Chang

来源：Journal of Food Biochemistry, 2018, 42(2): e12454.

DOI：10.1111/jfbc.12454

• anticancer
• antioxidant
• Eclipta alba
• Mesona procumbens Hemsl
• phenolic

This study investigated in vitro antioxidant and hepatocellular carcinoma (HepG2) cells growth inhibitory effect of Eclipta alba Hassk (EH) and Mesona procumbens Hemsl (MH). Extracts from ethanol 50% (E50) were chosen for the assays. Total phenolic and flavonoid content and the antioxidant activities including ABTS(+) and DPPH radical-scavenging system were determined. The identification of phenolic compounds was analyzed by high performance liquid chromatography (HPLC). It was found that higher levels of DPPH and ABTS(+) radical-scavenging activity were detected from the EH extract with IC50 of 41.910.08 and 75.49 +/- 1.15 mu g/mL, respectively. The rate of DPPH radical-scavenging activity was significantly influenced by the reaction time. The 4-hydroxybenzoic acid, caffeic acid, p-coumaric acid, and rosmarinic acid were the identified compounds in the EH. The 7-hydroxycoumarin, ferulic acid, and rutin being new compounds and two known compounds were identified in the MH. Lactate dehydrogenase released in medium was also evaluated. Extracts of the EH and MH stimulated the apoptotic factors bcl-2, bax, caspase-3, caspase-9 in HepG2. Overall, the EH extract was higher in total phenolic and flavonoid content, antioxidant potential and more effective in inhibiting cancer cell growth. @@@ Practical applicationsThe EH has been widely used for the treatment of sore throat, hair disorders, skin diseases, urinary tract infections, hematuria, and other diseases in traditional oriental medicine. Recently, in Vietnam the EH has also been used to successfully cure leukemia cancer. The MH is consumed as both a herbal drink and jelly-type dessert and it was used for antipyretic, heat-relieving, and lowering blood pressure. Both may be beneficial for the treatment of liver disease, such as liver cirrhosis, infectious hepatitis (liver inflammation due to a viral infection). This research revealed that the EH and MH may be good sources of potential phenolic phytochemicals that act as natural antioxidants and liver cancer cell growth inhibitor.

• 出版日期2018-4