Antiproliferative and immunoactivatory ability of an enzymatic extract from rice bran

作者：Revilla E; Santa Maria C; Miramontes E; Candiracci M; Rodriguez Morgado B; Carballo M; Bautista J; Castano A; Parrado J^*

来源：Food Chemistry, 2013, 136(2): 526-531.

DOI：10.1016/j.foodchem.2012.08.044

• Rice bran enzymatic extract
• Antiproliferative
• Cytotoxicity
• MOLT-4
• Immunoactivation
• Microglial cell
• TNF-alpha

The validation of natural products as source of functional foods or nutraceuticals has become an important issue in current health research. Thus, the present work has tested on MOLT-4 cells (human T cell acute lymphoblastic leukemic) the antiproliferative effect of a water-soluble enzymatic extract from rice bran (EERB). Present work shows that EERB induces cellular death in MOLT-4 cells in a dose-dependent way (0-10 mg/mL) but not in non-tumoral lymphocytes. Flow cytometric analysis of MOLT-4 cells treated with EERB showed the presence of death cells by apoptosis rather than necrosis. Additionally, EERB also exerts an immunoactivatory effect on N13 microglia cells, by inducing TNF-alpha (tumour necrosis factor-alpha) expression, which plays a key role in the innate immune response to infection. Accordingly, we can propose EERB as a useful natural standardized extract with antiproliferative and immunoactivatory ability that would be beneficial to apply in the functional food field.

• 出版日期2013-1-15