科研之友
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摘要
The nucleotide sequence of a DNA fragment, when present on a multi-copy plasmid, conferring ethionine resistance to Saccharomyces cerevisiae cells was determined. The fragment contained one long open reading frame and the frame was confirmed to be an ethionine resistant gene caused by frame-shift mutation. Other than ethionine resistance, an increased dosage of the gene directed overaccumulation in cells of S-adenosyl-L-methionine. The protein deduced from the open reading frame consisted of 617 amino acid residues with a calculated molecular weight of 67, 977.
- 出版日期1991
