Decreased CXCL12 is associated with impaired alveolar epithelial cell migration and poor lung healing after lung resection

作者：Kanter Jacob A; Sun Haiying; Chia Stephen; DeCamp Malcolm M; S**** Peter H S; Sznajder Jacob I; Bharat Ankit^*

来源：Surgery, 2015, 158(4): 1073-1080.

DOI：10.1016/j.surg.2015.04.051

Background. Prolonged air leak (PAL) is an important cause of morbidity and mortality after lung resection, but its pathogenesis has not been elucidated. Migration of alveolar type II epithelial cells is essential for lung wound repair. Here we determined the role of C-X-C motif chemokine 12 (CXCL12) on alveolar epithelial cell migration and lung wound healing.
Methods. CXCL12 in the pleural fluid of patients was analyzed using enzyme-linked immunosorbent assay. Human A549 and murine MLE12 alveolar epithelial cell lines were used for wound closure, cell migration, and proliferation assays. Western blot was used to analyze Rac1 and cofilin.
Results. Pleural CXCL12 was decreased in patients with PAL (1,389 +/- 192 vs 3,270 +/- 247 pg/mL; P < .0001). CXCL12 enhanced scratch wound closure in both A549 (77.9 +/- 0.7% vs 71.5 +/- 0.4%; P = .0016) and MLE12 (92.9 +/- 4.9% vs 66.0 +/- 4.8%; P = .017). CXCL12 enhanced migration by 57% in A549 (P = .0008) and by 86% in MLE12 (P < .0001). AMD3100, a selective CXCR4 antagonist, prevented the effects of CXCL12. CXCL12 increased Rac1 and cofilin activation but did not change bromodeoxyuridine incorporation or cell counts.
Conclusion. Reduced pleural CXCL12 is associated with PAL. CXCL12 promotes alveolar epithelial cell migration by binding to its receptor CXCR4 and may have a role in lung healing. CXCL12-mediated alveolar epithelial cell migration is associated with Rac1 and cofilin activation.

• 出版日期2015-10
• 单位西北大学