An evaluation of B-mode and color Doppler ultrasonography for detecting periovulatory events in the bitch

作者：Bergeron Lindsay H^*; Nykamp Stephanie G; Brisson Brigitte A; Madan Pavneesh; Gartley Cathy J

来源：Theriogenology, 2013, 79(2): 274-283.

DOI：10.1016/j.theriogenology.2012.08.016

• Canine
• Ovulation
• Ultrasound
• Color Doppler
• Preovulatory LH peak

When determining optimal breeding time in the bitch, specific periovulatory events must be identified. The main objectives were to relate ultrasonographic changes in ovarian blood flow, follicle/corpora lutea count and echotexture to periovulatory events, and to assess the efficacy of each for identifying these events. Twelve Beagle (N = 3), Beagle-cross (N = 2) and hound-cross (N = 7) bitches (body weight range, 7.5-27.5 kg) were examined daily from the onset of proestrus to approximately 4 days post-LH peak. Follicle and corpora lutea count and echotexture analyses were performed using B-mode ultrasound and ovarian blood flow analysis was performed using color Doppler ultrasound. Serum LH concentrations were analyzed by validated RIA. There was an increase (P < 0.05) in ovarian blood flow from the day of the preovulatory LH peak (605 pixels; confidence interval, 397856), to 1 day after this peak (1092 pixels; confidence interval, 72471535), enabling detection of the preovulatory LH peak. There were no significant changes in follicle/corpora lutea echotexture relative to days from the preovulatory LH peak. There were significant decreases in follicle/corpora lutea number between Days -1 and 3; Days -1 and 4; and Days 0 and 3, relative to the preovulatory LH peak. We concluded that color Doppler. ultrasound performed once daily was more accurate in identifying the preovulatory LH peak than B-mode ultrasound and enabled prospective determination of ovulation.

• 出版日期2013-1-15