New Alternative Analytical to Quantify the Anti-Leprosy Drug Dapsone by UV Spectrophotometry

作者：Araujo Rian F M; Coelho Mayara L; Soares Monica F L R; Rolim Larissa A; Neto Pedro J R; Soares Sobrinho Jose L^*

来源：Latin American Journal of Pharmacy, 2012, 31(2): 265-270.

• Analytical method
• Dapsone
• UV Spectrophotometry

Dapsone is the drug of choice for leprosy treatment. Despite the existence of previous analytical methods, this study aimed to develop a new analytical alternative for dapsone quantification by UV spectrophotometry. Since the pharmacopeial method uses methanol, a toxic solvent, the method was developed using first dilution in ethanol (500 mu g/mL) and second dilution in water (5 mu g/mL), with quantification in 295 nm. For validation, the specificity was confirmed by quantification of degraded samples using the developed method in comparison with a second analytical technique (HPLC-DAD). The proposed method proved to be specific, linear, precise, accurate and robust, with analytical low cost and toxicity, besides presenting operational ease.

• 出版日期2012-3