Antifungal resistance of Candida glabrata vaginal isolates and development of a quantitative reverse transcription-PCR-based azole susceptibility assay

作者:Gygax Scott E*; Vermitsky John Paul; Chadwick Sean G; Self Matthew J; Zimmerman Jessica A; Mordechai Eli; Adelson Martin E; Trama Jason P
来源:Antimicrobial Agents and Chemotherapy, 2008, 52(9): 3424-3426.
DOI:10.1128/AAC.00462-08

摘要

A multiplex quantitative reverse transcription-PCR assay was developed to detect azole resistance in Candida glabrata, an important opportunistic pathogen that develops resistance rapidly. Resistance was defined as a >= 3-fold increase in CDR1 expression by this assay, which proved to be 100% sensitive and 95% specific in comparison to the gold standard broth microdilution assay.

  • 出版日期2008-9