Analysis of receptor-ligand binding by photoaffinity cross-linking

作者：Xu Bin^*; Wu Ling

来源：Science China Chemistry, 2014, 57(2): 232-242.

DOI：10.1007/s11426-013-5045-z

• photoaffinity cross-linking
• receptor-ligand interaction
• photoscanning
• bifunctional photo-probe
• biosynthetic photo-probe
• novel receptor discovery

Photoaffinity cross-linking is a fast developing technology for biomolecular interactions, including receptor-ligand binding. The chemical mechanisms of the most commonly used photoactivatable probes and their respective photochemistry are summarized. This review focuses on the expanding utilities of this technology as a result of recent advances in the (i) identification of receptor contact sites, (ii) monitoring ligand-induced receptor conformational changes, (iii) identification of global binding surfaces, (iv) binding mode analysis using bifunctional photo-probes, (v) application of biosynthetic photo-probes, and (vi) examples of novel target discovery using this technology. Limitations and future potential of this approach are also discussed.

• 出版日期2014-2
• 单位Virginia Tech; 美国弗吉尼亚理工大学(Virginia Tech)