Fast human serum profiling through chemical depletion coupled to gold-nanoparticle-assisted protein separation

作者：Lopez Cortes Ruben; Oliveira Elisabete; Nunez Cristina; Lodeiro Carlos; Paez de la Cadena Maria; Fdez Riverola Fiorentino; Lopez Fernandez H; Reboiro Jato Miguel; Glez Pena Daniel; Luis Capelo Jose; Santos Hugo M

来源：Talanta, 2012, 100: 239-245.

DOI：10.1016/j.talanta.2012.08.020

• Medical diagnostics
• Serum profile
• MALDI
• Nanoparticles
• Nanomedicine
• Chemical depletion

The use of chemical protein depletion in conjunction with gold-based nanoparticles for fast matrix assisted laser desoption ionization time of flight mass spectrometry-based human serum profiling was assessed. The following variables influencing the process were optimized: (i) amount of nanoparticles, (ii) sample pH, (iii) amount of protein and (iv) incubation time. pH was found the most important factor to be controlled, with an optimum range comprised between 5.8 and 6.4. The minimum incubation time to obtain an adequate profiling was 30 min. Using this approach, serum from five patients with lymphoma, five patients with myeloma and from two healthy volunteers were correctly classified using Principal component analysis.

• 出版日期2012-10-15