An amperometric biosensor based on laccase immobilized onto Fe(3)O(4)NPs/cMWCNT/PANI/Au electrode for determination of phenolic content in tea leaves extract

作者：Rawal Rachna; Chawla Sheetal; Pundir Devender C S^*

来源：Enzyme and Microbial Technology, 2012, 51(4): 179-185.

DOI：10.1016/j.enzmictec.2012.06.001

• Phenolic compounds
• Laccase
• Amperometric biosensor
• Canoderma sp.
• Fe(3)O(4)NPs
• MWCNT
• PANI
• Au electrode

A method is described for the construction of an amperometric biosensor for detection of phenolic compounds based on covalent immobilization of laccase onto iron oxide nanoparticles (Fe(3)O(4)NPs) decorated carboxylatecl multiwalled carbon nanotubes (cMWCNTs)/polyaniline (PANI) composite electrodeposited onto a gold (Au) electrode. The modified electrode was characterized by scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The biosensor showed optimum response within 3 s at pH 6.0 (0.1 M sodium acetate buffer) and 35 degrees C. when operated at 0.3 V vs. Ag/AgCl. Linear range, detection limit were 0.1-10 mu M (lower concentration range) and 10-500 mu M (higher concentration range), and 0.03 mu M respectively. The sensor measured total phenolic content in tea leaves extract. The enzyme electrode lost 25% of its initial activity after its 150 uses over a period of 4 months, when stored at 4 degrees C.

• 出版日期2012-9-10