Neuron-Specific Expression of Tomosyn1 in the Mouse Hippocampal Dentate Gyrus Impairs Spatial Learning and Memory

作者：Barak Boaz; Okun Eitan; Ben Simon Yoav; Lavi Ayal; Shapira Ronit; Madar Ravit; Wang Yue; Norman Eric; Sheinin Anton; Pita Mario A; Yizhar Ofer; Mughal Mohamed R; Stuenkel Edward; van Praag Henriette; Mattson Mark P; Ashery Uri^*

来源：NeuroMolecular Medicine, 2013, 15(2): 351-363.

DOI：10.1007/s12017-013-8223-4

• Synaptic plasticity
• Synaptic transmission
• Behavior
• Tomosyn
• Short-term plasticity
• Hippocampus
• Lentivirus
• Dentate gyrus

Tomosyn, a syntaxin-binding protein, is known to inhibit vesicle priming and synaptic transmission via interference with the formation of SNARE complexes. Using a lentiviral vector, we specifically overexpressed tomosyn1 in hippocampal dentate gyrus neurons in adult mice. Mice were then subjected to spatial learning and memory tasks and electrophysiological measurements from hippocampal slices. Tomosyn1-overexpression significantly impaired hippocampus-dependent spatial memory while tested in the Morris water maze. Further, tomosyn1-overexpressing mice utilize swimming strategies of lesser cognitive ability in the Morris water maze compared with control mice. Electrophysiological measurements at mossy fiber-CA3 synapses revealed impaired paired-pulse facilitation in the mossy fiber of tomosyn1-overexpressing mice. This study provides evidence for novel roles for tomosyn1 in hippocampus-dependent spatial learning and memory, potentially via decreased synaptic transmission in mossy fiber-CA3 synapses. Moreover, it provides new insight regarding the role of the hippocampal dentate gyrus and mossy fiber-CA3 synapses in swimming strategy preference, and in learning and memory.

• 出版日期2013-6
• 单位NIH