A Triple Arg Motif Mediates a2B-Adrenergic Receptor Interaction with Sec24C/D and Export

作者：Dong Chunmin; Nichols Charles D; Guo Jianhui; Huang Wei; Lambert Nevin A; Wu Guangyu^*

来源：Traffic, 2012, 13(6): 857-868.

DOI：10.1111/j.1600-0854.2012.01351.x

• adrenergic receptor
• cell surface transport
• COPII vesicle
• endoplasmic reticulum
• export motif
• G protein-coupled receptor
• intracellular loop
• Sec24

Recent studies have demonstrated that cargo exit from the endoplasmic reticulum (ER) may be directed by ER export motifs recognized by components of the coat protein II (COPII) vesicles. However, little is known about ER export motifs and vesicle targeting of the G protein-coupled receptor (GPCR) superfamily. Here, we have demonstrated that a triple Arg (3R) motif in the third intracellular loop functions as a novel ER export signal for a2B-adrenergic receptor (a2B-AR). The 3R motif mediates a2B-AR interaction with Sec24C/D and modulates ER exit, cell surface transport and function of a2B-AR. Furthermore, export function of the 3R motif is independent of its position within a2B-AR and can be conferred to CD8 glycoprotein. These data provide the first evidence implicating that export of GPCRs is controlled by code-directed interactions with selective components of the COPII transport machinery.

• 出版日期2012-6