Anti-Inflammatory and Anti-Melanogenic Steroidal Saponin Glycosides from Fenugreek (Trigonella foenum-graecum L.) Seeds

作者：Kawabata Tetsuro; Cui Ming Yue; Hasegawa Tatsuya; Takano Fumihide^*; Ohta Tomihisa

来源：Planta Medica, 2011, 77(7): 705-710.

DOI：10.1055/s-0030-1250477

• Trigonella foenum-graecum
• Fabaceae
• anti-inflammatory
• melanogenesis
• steroidal saponin

Fenugreek seed (Trigonella foenum-graecum L.) is used as an herbal medicine for treating metabolic and nutritive dysfunctions. To determine if this plant has other beneficial effects, we tested the inhibitory activities of a methanol (MeOH) extract of fenugreek seed on the production of inflammatory cytokines and melanin synthesis in cultured cell lines in vitro. The MeOH extract inhibited the production of phorbol-12-myristate-13-acetate-induced inflammatory cytokines such as tumor necrosis factor (TNF)-alpha in cultured THP-1 cells, and also restrained the intracellular synthesis of melanin in murine melanoma B16F1 cells. We isolated three active constituents from fenugreek seed extracts. These were identified as the steroidal saponins 26-O-beta-D-glucopyranosyl-(25R)-furost- 5(6)-en-3 beta,22 beta,26-triol-3-O-alpha-L-rhamnopyranosyl-(1 ''-> 2')-O-[beta-D-glucopyranosyl-(1'''-> 6')-O]-beta-D-glucopyranoside 1, minutoside B 2, and pseudoprotodioscin 3. Compounds 1 and 2 strongly suppressed the production of inflammatory cytokines, whereas 3 showed a weaker suppressing effect. Melanogenesis in B16F1 cells was significantly suppressed by 1 and 3, and weakly suppressed by 2. All three compounds showed moderate cytotoxicities. These results indicate that fenugreek extract and its active constituents could protect against skin damage.

• 出版日期2011-5