Antiviral effect of recombinant equine interferon-gamma on several equine viruses

作者：Sentsui, Hiroshi^*; Wu, Donglai; Murakami, Kenji; Kondo, Takashi; Matsumura, Tomio

来源：Veterinary Immunology and Immunopathology, 2010, 135(1-2): 93-99.

DOI：10.1016/j.vetimm.2009.11.006

• Baculovirus
• Equine virus
• Horse
• Interferon-gamma
• Recombinant interferon

Recombinant equine interferon-gamma (reIFN-gamma) was prepared using a baculovirus expression system and its antiviral activity was investigated using several equine viruses. The reIFN-gamma suppressed the replication of all equine viruses used in the present experiment in horse cell cultures, but did not affect the growth of host cells at concentrations of less than 1000 u/ml. A strong antiviral effect was observed, especially against RNA viruses. Equine picornavirus, equine rhinovirus and equine arteritis virus could not be propagated at all in 100 u/ml reIFN-gamma when 100 TCID(50) of infective viruses was inoculated to cultivated horse cells. DNA viruses, equine herpesvirus types 1, 2, 3 and 4 and equine adenovirus, were less sensitive to reIFN-gamma but their growth became less than 1/100 in the cells treated with 100 u/ml reIFN-gamma compared to untreated cells. The antiviral effects were decreased in the cells of heterologous species and more than 1000 u/ml reIFN-gamma was required to induce an antiviral effect.

• 出版日期2010-5-15
• 单位中国农业科学院; 中国农业科学院哈尔滨兽医研究所