Aggressive mature natural killer cell neoplasms: report on a series of 12 European patients with emphasis on flow cytometry based immunophenotype and DNA content of neoplastic natural killer cells

作者：Lima Margarida^*; Spinola Ana; Fonseca Sonia; Santos Ana Helena; Rodrigues Joao; Oliveira Lurdes; Queiros Maria Luis; Santos Marlene; Goncalves Marta; Lau Catarina; Teixeira Maria dos Anjos; Goncalves Cristina; Marques Cristina; Guerreiro Manuel; Cunha Manuel; Principe Fernando; Coutinho Jorge

来源：Leukemia and Lymphoma, 2015, 56(1): 103-112.

DOI：10.3109/10428194.2014.905772

• Aggressive NK cell leukemia
• NK/T cell lymphoma
• nasal type
• flow cytometry
• NK cell immunophenotyping
• CD56+bright NK cells
• DNA content and cell cycle

We report 12 cases of aggressive natural killer (NK) cell neoplasms diagnosed in Portugal, with emphasis on flow cytometry. Ten patients had extranodal NK/T cell lymphoma, nasal type and two had aggressive NK cell leukemia, and seven were men and five were women, with a median age of 50 years. NK cells brightly expressed the CD56 adhesion molecule and CD94 lectin type killer receptor and had an activation-related HLA-DR. CD45RA. CD45RO. immunophenotype, in most cases. In contrast, dim CD16 expression was found in a minor proportion of cases, whereas CD57 and the CD158a and CD158e1 killer immunoglobulin-like receptors were negative. One-third of cases showed a hyperploid DNA content and nearly all had a very high S-phase proliferative rate. The phenotypic features of the neoplastic NK cells would suggest that they represent the transformed counterpart of the CD56. bright NK cells that circulate in normal blood.

• 出版日期2015-1