Acceleration of Anaerobic Cysteine Transformations to Sulfane Sulfur Consequent to gamma-Glutamyl Transpeptidase Inhibition

作者：Kwiecien Inga^*; Iciek Malgorzata; Wlodek Lidia

来源：The Scientific World Journal, 2012, 253724.

DOI：10.1100/2012/253724

Toxicity of drugs and radiation in the cells is largely dependent on the level of thiols. In the present studies, an attempt has been made to inhibit gamma-glutamyl transpeptidase (gamma GT) activity in EAT-bearing animals tissue. We have expected that administration of gamma GT inhibitors: acivicin and 1,2,3,4-tetrahydroisoquinoline (TIQ) may influence GSH/gamma-glutamyl transpeptidase (gamma GT) system in the regulation of cysteine concentration and anaerobic cysteine metabolism in normal and cancer cells. Development of Ehrlich ascites tumor in mice enhances peroxidative processes, diminishes levels of nonprotein thiols (NPSH) and sulfane sulfur, and lowers activities of enzymes involved in its formation and transfer in the liver and kidney. Although gamma GT inhibitors further decrease NPSH level, they increase cysteine and sulfane sulfur levels. This means that upon gamma GT inhibition, cysteine can be efficiently acquired by normal liver and kidney cells via another pathway, that is so productive that sulfane sulfur level and intensity of anaerobic cysteine metabolism even rise.

• 出版日期2012