Highly sensitive and selective spectrofluorimetric determination of nitrite in food products with a novel fluorogenic probe

作者：Wang, Qiuhua; Ma, Sufang; Huang, Haiwei; Cao, Aocheng^*; Li, Minfeng^*; He, Lan^*

来源：Food Control, 2016, 63: 117-121.

DOI：10.1016/j.foodcont.2015.11.023

• Nitrite
• Spectrofluorimetric
• Determination
• Dihydropyridine

A simple, sensitive, selective and convenient spectrofluorimetric method for detection of nitrite has been successfully developed. This method is based on the highly efficient and specific reaction of nitric oxide with a dihydropyridine derivative to form a highly fluorescent pyridine derivative, in aqueous solution. Under optimum conditions, excellent detection linear range was achieved from 0.2 to 4.5 mu mol L-1 with a detection limit of 0.021 mu mol L-1 (S/N = 3), lower than or comparable to most of other spectrofluorimetric methods. The relative standard deviation for ten determinations of 2.0 mu mol L-1 nitrite was 1.0%. The proposed method has been applied to determine nitrite levels in food products with recoveries of 93.3%-103.5%.

• 出版日期2016-5
• 单位中国农业科学院; 北京师范大学