科研之友
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摘要
Interspecific hybridization in roses (Rosa spp.) has generated thousands of cultivars with commercial value, but only ten species are commonly represented in the pedigrees of modern cultivars. This would suggest that unexploited wild rose species have the potential to broaden the overall gene pool of rose breeding programs in general. We utilized previously described microsatellite (SSR) markers to generate DNA profiles in order to assess the genetic diversity of 44 selected rose genotypes among which are represented wild species, miniature roses and interspecific hybrids. A total of 83 alleles were scored using 15 SSR loci. The number of distinct alleles per locus ranged from three to eight, with an average of 5.5 per locus. Genetic distance matrices were used to generate dendrogram based on the procedure of minimum variance or Ward's method of clustering using the Multivariate Statistical Package. Cluster analysis groupings for the 44 genotypes segregated into three major categories which is consistent with taxonomy and their respective ploidy levels with only a few exceptions. Similarity coefficients determined for wild rose populations had a lower average value of 0.66 (ranged from 0.47 to 0.98) than those calculated for domesticated miniature roses and hybrid breeding lines which had an average value of 0.73 (ranged from 0.57 to 0.83). This indicates that modern rose hybrids have a narrower genetic base than wild relatives, and could benefit from the integration of valuable wild rose germplasm into the efforts of the hybrid rose breeding profession. Published by Elsevier B.V.
- 出版日期2012-11-12
- 单位东北林业大学
