科研之友
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摘要
Fine-tuning emission fluorescent bioprobes are highly desirable but still scarce for dual-alternating-color live cell imaging. Herein, a facile strategy has been reported to develop a dual-color nanoprobe for targeted cancer cell imaging. A new kind of nonconjugated polymer dots (Pdots) is prepared by the solution self-assembly of an amphiphilic 4-arm star polymer TPE-tetraPNIPAM-Eu(III) in aqueous media. The as-prepared Pdots show two well-separated emission peaks with a wavelength difference of 195 nm and possess a fluorescent-thermoresponsive property with a LCST at 37 degrees C. Furthermore, the efficient cellular uptake and low cytotoxicity of Pdots can be favorable for their applications in live cell imaging. The investigation of cellular imaging indicates that the photoswitchable dual-emission could be easily realized in targeted three cancer cells with a fluorescent label of Pdots by merely tuning the excitation wavelength. Therefore, this research may be of great help to open up a new and feasible path of developing AIE nonconjugated Pdots biomaterials for multicolor cell imaging applications.
