Mitochondrial genomes of Trichinella species and genotypes - a basis for diagnosis, and systematic and epidemiological explorations

作者：Mohandas Namitha; Pozio Edoardo; La Rosa Giuseppe; Korhonen Pasi K; Young Neil D; Koehler Anson V; Hall Ross S; Sternberg Paul W; Boag Peter R; Jex Aaron R; Chang Bill C H; Gasser Robin B^*

来源：International Journal for Parasitology, 2014, 44(14): 1073-1080.

DOI：10.1016/j.ijpara.2014.08.010

• Trichinella/trichinellosis
• Deep sequencing
• Bioinformatics
• Mitochondrial genome
• Genetic relationships
• Systematics
• Population genetics
• Epidemiology

In the present study we sequenced or re-sequenced, assembled and annotated 15 mitochondrial genomes representing the 12 currently recognised taxa of Trichinella using a deep sequencing-coupled approach. We then defined and compared the gene order in individual mitochondrial genomes (similar to 14 to 17.7 kb), evaluated genetic differences among species/genotypes and re-assessed the relationships among these taxa using the mitochondrial nucleic acid or amino acid sequence data sets. In addition, a rich source of mitochondrial genetic markers was defined that could be used in future systematic, epidemiological and population genetic studies of Trichinella. The sequencing-bioinformatic approach employed herein should be applicable to a wide range of eukaryotic parasites.

• 出版日期2014-12