Utility of next-generation sequencing methods to identify the novel HLA alleles in potential stem cell donors from Chinese Marrow Donor Program

作者：Qi Jun^*; Wang Tian Ju; Chen Li Ping; Wang Xiao Fang; Wang Man Ni; Wu Jun Hua

来源：International Journal of Immunogenetics, 2018, 45(4): 225-229.

DOI：10.1111/iji.12377

• human leucocyte antigen
• next-generation sequencing
• novel allele
• sequencing-based typing

The human leucocyte antigen (HLA) is the most polymorphic region of the human genome. Compared with Sanger-sequencing-based typing (SBT) methods, next-generation sequencing (NGS) has significantly higher throughput and depth sequencing characteristics, having dramatic impacts on HLA typing in clinical settings. Here, we performed NGS technology with Ion Torrent S5 platform to evaluate the potential four novel HLA alleles detected in five donors from Chinese Marrow Donor Program (CMDP, Shaanxi Province) during routine Sanger SBT testing. We also predicted the highest estimated relative frequency novel allele-bearing haplotypes according to their phenotypes and HaploStats database. NGS assays, as it provided the phase-defined and complete sequencing information, undoubtedly increase novel allele identification which will greatly enrich HLA database and provide more information for donor selection.

• 出版日期2018-8
• 单位陕西省血液中心