Anti-inflammatory effects of glycyrol isolated from Glycyrrhiza uralensis in LPS-stimulated RAW264.7 macrophages

作者：Shin Eun Myoung; Zhou Hong Yu; Guo Lian Yu; Kim Jeong Ah; Lee Seung Ho; Merfort Irmgard; Kang Sam Sik; Kim Hak Sung; Kim Sanghee; Kim Yeong Shik^*

来源：International Immunopharmacology, 2008, 8(11): 1524-1532.

DOI：10.1016/j.intimp.2008.06.008

• Glycyrrhizae Radix
• Glycyrol
• Anti-inflammatory activity
• NF-kappa B
• iNOS
• COX-2

The anti-inflammatory effects of glycyrol, a benzofuran coumarin isolated from Glycyrrhizae Radix, were studied. Glycyrol of 5, 25 and 50 mu M dose-dependently inhibited nitric oxide (NO) production by down-regulating inducible nitric oxide synthase (iNOS), and alleviated cyclooxygenase-2 (COX-2) expression in LPS-stimutated RAW264.7 macrophages, in both the mRNA and the protein. Furthermore, glycyrol dose-dependently decreased the mRNA of the pro-inflammatory cytokines IL-1 beta and IL-6. LPS-induced NF-kappa B activation was prevented in RAW264.7 macrophages by inhibition of I-kappa B alpha phosphorylation. In addition, administration of glycyrol (30 and 100 mg/kg, i.p) reduced the thickness of carrageenan-induced mouse-paw edema swelling. Taken together, our results indicate that glycyrol is an important anti-inflammatory constituent of Glycyrrhizae Radix, and that its anti-inflammatory effect is attributed to the inhibition I-kappa B alpha phosphorylation.

• 出版日期2008-11
• 单位沈阳药科大学