A comprehensive two-dimensional normal-phase x reversed-phase liquid chromatography based on the modification of mobile phases

作者：Wei Yuanlong; Lan Tao; Tang Tao; Zhang Lingyi; Wang Fengyun; Li Tong; Du Yiping; Zhang Weibing^*

来源：Journal of Chromatography A, 2009, 1216(44): 7466-7471.

DOI：10.1016/j.chroma.2009.08.029

• Two-dimensional LC
• Mobile phase modification
• Tandem-column
• Sample loop-valve interface
• Zhengtian pill

A comprehensive orthogonal two-dimensional liquid chromatography (2D-LC) based oil the modification of mobile phases was developed with a sample loop-valve interface. To improve the compatibility of mobile phases and analysis speed. some special solvents were chosen as the mobile phases, and the column temperature was elevated to decrease the viscosity or mobile phases of reversed-phase liquid chromatography(RPLC). Based oil this principle, the first dimension was normal-phase liquid chromatography (NPLC) with a SiO(2) column, and the second dimension was reversed-phase liquid chromatography containing two tandem C18 columns 1,4-Dioxane was used in the NPLC mobile phase, and isopropyl alcohol was employed in the RPLC mobile phase. Moreover, the elevated column temperature enabled the reduction of the backpressure and using tandem C18 columns to improve the resolving power in RPLC. The new comprehensive 2D-LC system and applied strategy offered a novel idea for construction of 2D-LC system A traditional Chinese medicine. Zhengtian pill, was used as the test sample to evaluate the constructed 2D-LC system. 876 peaks wet e detected. and the peak capacity reached 1740.

• 出版日期2009-10-30
• 单位华东理工大学; 南京理工大学