MPTP related mitochondrial pathway in oroxylin A induced-apoptosis in HepG2 cancer cells

作者：Huang, Xin-Eng; Wei, Da; Yang, Yi-Ning; Chen, Sen-Qing; Zhu, Ming; Zhang, Xiao-Mei; Yu, Jun^*

来源：International Journal of Clinical and Experimental Pathology, 2016, 9(11): 11139-11148.

• MPTP
• oroxylin A
• ROS
• apoptosis

Oroxylin A is a flavonoid extracted from the root of Scutellaria baicalensis Georgi. Our previous work demonstrated that oroxylin A induced cell apoptosis via the mitochondrial pathway in cancer cells. The present study explored the mechanism underlying oroxylin A-induced apoptosis. We firstly showed that oroxylin A caused apoptosis through the mitochondrial pathway in human hepatoma HepG2 cells. We next demonstrated that mitochondrial permeability transition pore (MPTP) was activated, suggesting the elevated permeabilization of the mitochondrial outer membrane after oroxylin A treatment. These data indicated that Oroxylin A-induced apoptosis via a MPTP-dependent manner. The production of reactive oxygen species (ROS) is essential for the activation of MPTP, and we confirmed that Oroxylin A increased MPTP activation in a ROS-dependent manner. We finally showed that Oroxylin A inhibited the proliferation of HepG2 xerograph in vivo. Collectively, our results demonstrated that oroxylin A induced apoptosis by activating MPTP in human hepatoma cells.

• 出版日期2016
• 单位南京医科大学; 江苏省肿瘤防治研究所