In vivo fate tracking of degradable nanoparticles for lung gene transfer using PET and Cerenkov imaging

作者：Black Kvar C L; Ibricevic Aida; Gunsten Sean P; Flores Jeniree A; Gustafson Tiffany P; Raymond Jeffery E; Samarajeewa Sandani; Shrestha Ritu; Felder Simcha E; Cai Tianyi; Shen Yuefei; Lobs Ann Kathrin; Zhegalova Natalia; Sultan Deborah H; Berezin Mikhail; Wooley Karen L; Liu Yongjian; Brody Steven L

来源：Biomaterials, 2016, 98: 53-63.

DOI：10.1016/j.biomaterials.2016.04.040

• Degradable nanoparticle
• Biodistribution
• Cytotoxicity
• Lung
• Gene expression
• Cerenkov luminescence imaging

Nanoparticles (NPs) play expanding roles in biomedical applications including imaging and therapy, however, their long-term fate and clearance profiles have yet to be fully characterized in vivo. NP delivery via the airway is particularly challenging, as the clearance may be inefficient and lung immune responses complex. Thus, specific material design is required for cargo delivery and quantitative, noninvasive methods are needed to characterize NP pharmacokinetics. Here, biocompatible poly(acrylamidoethylamine)-b-poly(DL-lactide) block copolymer-based degradable, cationic, shell-cross-linked knedel-like NPs (Dg-cSCKs) were employed to transfect plasmid DNA. Radioactive and optical beacons were attached to monitor biodistribution and imaging. The preferential release of cargo in acidic conditions provided enhanced transfection efficiency compared to non-degradable counterparts. In vivo gene transfer to the lung was correlated with NP pharmacokinetics by radiolabeling Dg-cSCKs and performing quantitative biodistribution with parallel positron emission tomography and cerenkov imaging. Quantitation of imaging over 14 days corresponded with the pharmacokinetics of NP movement from the lung to gastrointestinal and renal routes, consistent with predicted degradation and excretion. This ability to noninvasively and accurately track NP fate highlights the advantage of incorporating multifunctionality into particle design.

• 出版日期2016-8