A modification of intraluminal middle cerebral artery occlusion/reperfusion model for ischemic stroke with laser Doppler flowmetry guidance in mice

作者：Cai, Qiang; Xu, Gang; Liu, Junhui; Wang, Long; Deng, Gang; Liu, Jun; Chen, Zhibiao^*

来源：Neuropsychiatric Disease and Treatment, 2016, 12: 2851-2858.

DOI：10.2147/NDT.S118531

• middle cerebral artery occlusion
• ischemia stroke
• animal model
• mice

Stroke is one of the common causes of death and disability in the world. The intraluminal middle cerebral artery occlusion/reperfusion (MCAO/R) model is a "gold standard" in surgical ischemic stroke models. Here, we optimized the procedure of this model by ligating on external carotid artery (ECA) stump and two ligatures prepared on internal carotid artery, which could improve the success and survival rate in mice. The results show that ECA approach was superior to common carotid artery approach. Meanwhile, we found that the exposure of pterygopalatine artery was not an essential step for MCAO/R model in mice.

• 出版日期2016
• 单位武汉大学; 武汉市中心医院