Mpp7 controls regioselective Knoevenagel condensation during the biosynthesis of Monascus azaphilone pigments

作者：Balakrishnan Bijinu; Chen Chien Chi; Pan Tzu Ming; Kwon Hyung Jin^*

来源：Tetrahedron Letters, 2014, 55(9): 1640-1643.

DOI：10.1016/j.tetlet.2014.01.090

• Monascus azaphilone pigment biosynthesis
• Monascus purpureus
• Knoevenagel aldol condensation
• Targeted inactivation of mpp7

Targeted inactivation of the mpp7 gene in the Monascus azaphilone pigment (MAzP) biosynthetic gene cluster resulted in the accumulation of monasfluol A (7) and B (8), of which the latter was a novel compound, and the abolition of the main MAzPs. It is thus proposed that 7 and 8 are the products of nonenzymatic Knoevenagel condensation followed by a reduction and that Mpp7 assists in regioselective Knoevenagel aldol condensation during MAzP biosynthesis.

• 出版日期2014-2-26