Atomic-resolution structures from fragmented protein crystals with the cryoEM method MicroED

作者：de la Cruz M Jason; Hattne Johan; Shi Dan; Seidler Paul; Rodriguez Jose; Reyes Francis E; Sawaya Michael R; Cascio Duilio; Weiss Simon C; Kim Sun Kyung; Hinck Cynthia S; Hinck Andrew P; Calero Guillermo; Eisenberg David; Gonen Tamir^*

来源：Nature Methods, 2017, 14(4): 399-+.

DOI：10.1038/nmeth.4178

Traditionally, crystallographic analysis of macromolecules has depended on large, well-ordered crystals, which often require significant effort to obtain. Even sizable crystals sometimes suffer from pathologies that render them inappropriate for high-resolution structure determination. Here we show that fragmentation of large, imperfect crystals into microcrystals or nanocrystals can provide a simple path for high-resolution structure determination by the cryoEM method MicroED and potentially by serial femtosecond crystallography.

• 出版日期2017-4