Association between adiponectin gene polymorphisms and obesity in school age children from Hualpen, Chile

作者：Orellana Gloria; Sapunar Jorge; Saez Katia; Aguayo Claudio; Calvo Carlos; Radojkovic Claudia; Riffo Benilde; Gleisner Andrea; Asenjo Sylvia; Ulloa Natalia

来源：Revista Medica de Chile, 2012, 140(10): 1245-1252.

DOI：10.4067/S0034-98872012001000002

• apM-1 protein
• Child
• Obesity
• Polymorphisms, Single Nucleotide

Background: Several genetic polymorphisms of adiponectin have been associated to metabolic diseases as obesity and co-morbidities. Aim: To investigate if there are associations between +45TG, +276GT, -11,377CG y -11,391GA adiponectin SNPs (single nucleotide polymorphism) with obesity in a Chilean children population. Material and Methods: A case-control study was performed in 241 obese and 126 normal weight children (7-11 years old) from the urban community of Hualpen, Biobio region. Children were classified as normal or obese, according to age and gender-specific percentiles defined by Center for Disease Control and Prevention (CDC). The analysis of serum markers was carried out using commercial kits. Adiponectin polymorphisms were determined through a High Resolution Melting (HRM)-enabled real time PCR and by DNA fragment sequencing. Results: The observed allelic frequencies of the studied SNPs were over 11%. The 11,377CG polymorphism was associated with a high risk of obesity, calculated by the additive inheritance model (odds ratio = 1.389, 95% confidence interval: 1.001-1.929, p = 0.049). Conclusions: Obese school children of the Biobio Region, have an increased risk of carrying the susceptibility allele polymorphism 11377CG of adiponectin gene. (Rev Med Chile 2012; 140: 1245-1252).

• 出版日期2012-10