Application of continuous supercritical anti-solvents for rapid recrystallization and purification of zeaxanthin dipalmitates from de-glycosides of Lycium barbarum fruits

作者：Chang Li Pin; Cheng Jia Hui; Hsu Shih Lan; Liau Bing Chung; Wu Tzong Ming; Chang Chieh Ming J^*

来源：Journal of Supercritical Fluids, 2011, 57(2): 155-161.

DOI：10.1016/j.supflu.2011.02.008

• Lycium barbarum
• Zeaxanthin palmitates
• Supercritical anti-solvent recrystallization
• Anti-oxidative ability
• Human adult retinal pigment epithelial cells

This study employed column elution fractionation coupled with supercritical anti-solvent recrystallization to isolate the purest zeaxanthin dipalmitates (ZP), one of the eye-protecting compounds in the Lycium barbarum fruits. The amount of ZP increased to 983 mg/gin liquid anti-solvent precipitates with 29.1% recovery and increased to 929 mg/gin supercritical anti-solvent precipitates with 59.6% recovery, respectively from the ultrasonic acetone extract. Experimental data showed that supercritical anti-solvent recrystallization is feasible in producing platelet type precipitates within a few minutes. These ZP compounds exhibit anti-oxidative abilities by proliferating human adult retinal pigment epithelial cells (from 22% to 31%); though it was a meager increase when compared to those of algal 95% zeaxanthin (from 43% to 94%).

• 出版日期2011-6