A Direct Comparison of Expression Profiles of Adhesion Molecules on Naive T Cells Between Cord Blood and Steady-State Bone Marrow Grafts of Healthy Donors

作者：Sun, L. -X.^*; Li, Y. -Q.; Song, X. -N.; Jiang, J.; Chang, Y. -J.

来源：Transplantation Proceedings, 2013, 45(1): 415-419.

DOI：10.1016/j.transproceed.2012.05.092

• VERSUS-HOST-DISEASE
• HEMATOPOIETIC STEM-CELLS
• LYMPHOCYTE SUBSETS
• PEDIATRIC-PATIENTS
• TRANSPLANTATION
• METAANALYSIS
• STIMULATION
• ACTIVATION
• LEUKEMIA
• SIGNALS

Introduction. We compared the profiles of adhesion molecule expression on naive T cells between umbilical cord blood (UCB) and steady-state bone marrow (SS-BM) grafts.
Methods. The expressions of 4 adhesion molecules, including very late antigen 4 (VLA-4), intercellular adhesion molecule-1 (ICAM-1), L-selectin, and lymophocyte function-associated antigen-1 (LFA-1) on naive T cells in UCB (n = 25) and SS-BM (n = 10) were analyzed using flow cytometry.
Results. The expressions of ICAM-1 and L-selectin on CD4(+) T cells and CD8(+) T cells in UCB were significantly lower than those on SS-BM (P < .05 for all). The expressions of VLA-4 and LFA-1 on CD8(+) T cells in UCB were significantly lower than those of SS-BM (P = .002 and .047, respectively). Compared with SS-BM, we observed lower expression of ICAM-1 on naive CD4(+) and CD8(+) T cells in UCB (P < .001 for all). The percentages of interferon (IFN)-gamma positive cells among naive CD4(+) and CD8(+) T-cell subsets were significantly lower in UCB, leading to ready polarization of naive UCB T cells from a Th1 to Th2 phenotype versus those on SS-BM.
Conclusions. Our results among UCB suggested lower intensities of ICAM-1 expression on naive T cells and their easier polarization from Th1 to Th2 elements.

• 出版日期2013-2
• 单位河北医科大学