A New Gene Expression Signature for Triple-Negative Breast Cancer Using Frozen Fresh Tissue before Neoadjuvant Chemotherapy

作者：Santuario Facio Sandra K; Cardona Huerta Servando; Perez Paramo Yadira X; Trevino Victor; Hernandez Cabrera Francisco; Rojas Martinez Augusto; Uscanga Perales Grecia; Martinez Rodriguez Jorge L; Martinez Jacobo Lizeth; Padilla Rivas Gerardo; Munoz Maldonado Gerardo; Francisco Gonzalez Guerrero Juan; Valero Gomez Javier; Vazquez Guerrero Ana L; Martinez Rodriguez Herminia G; Barboza Quintana Alvaro; Barboza Quintana Oralia; Garza Guajardo Raquel; Ortiz Lopez Rocio

来源：Molecular Medicine, 2017, 23(1): 1.

DOI：10.2119/molmed.2016.00257

Triple-negative breast cancer (TNBC) is an aggressive subtype of breast cancer tumors. Comparisons between TNBC and non-triple-negative breast cancer (nTNBC) may help to differentiate key components involved in TNBC neoplasms. The purpose of the study was to analyze the expression profile of TNBC versus nTNBC tumors in a homogeneous population from northeastern Mexico. A prospective study of 50 patients (25 TNBC and 25 nTNBC) was conducted. Clinic parameters were equally distributed for TNBC and nTNBC: age at diagnosis (51 versus 47 years, p = 0.1), glucose level (107 mg/dl versus 104 mg/dl, p = 0.64), and body mass index (28 versus 29, p = 0.14). Core biopsies were collected for histopathological diagnosis and gene expression analysis. Total RNA was isolated and expression profiling was performed. Forty genes showed differential expression pattern in TNBC tumors. Among these, nine overexpressed genes (PRKX/PRKY, UGT8, HMGA1, LPIN1, HAPLN3, FAM171A1, BCL141A, FOXC1, and ANKRD11), and one underexpressed gene (ANX9) are involved in general metabolism. Based on this biochemical peculiarity and the overexpression of BCL11A and FOXC1 (involved in tumor growth and metastasis, respectively), we validated by quantitative polymerase chain reaction the expression profiles of seven genes out of the signature. In this report, a new gene signature for TNBC is proposed. To our knowledge, this is the first TNBC signature that describes genes involved in general metabolism. The findings may be pertinent for Mexican patients and require evaluation in other ethnic groups and populations.

• 出版日期2017