MR spectroscopy measurement of the diffusion of dimethyl sulfoxide in articular cartilage and comparison to theoretical predictions

作者：Abazari A; Elliott J A W; McGann L E; Thompson R B^*

来源：Osteoarthritis and Cartilage, 2012, 20(9): 1004-1010.

DOI：10.1016/j.joca.2012.04.023

• Magnetic resonance imaging
• Spectroscopy
• Dimethyl sulfoxide diffusion
• Cartilage
• Biomechanical model

Cartilage cryopreservation requires optimal loading of protective solutes, most commonly dimethyl sulfoxide (DMSO), to maximize chondrocyte survival. Previously, diffusion models have been used to predict the distribution of solutes in tissue samples, but the accuracy of spatiotemporal predictions of these models have not been validated with empirical studies and remains unknown.
Objective: In this study, magnetic resonance spectroscopic imaging was used to measure the spatial and temporal changes in DMSO and water concentrations in porcine articular cartilage plugs, throughout 1 h of solute loading.
Design: A custom NMR spectroscopic imaging pulse sequence provided water and DMSO concentration images with an in-plane spatial resolution of 135 mu m and a temporal resolution of 150 s, repeated for 60 min throughout DMSO loading. Delayed gadolinium-enhanced magnetic resonance of cartilage (d-GEMRIC) imaging provided fixed charge density and spin-density imaging provided water density images prior to DMSO loading.
Results: The measured spatial and temporal distribution of DMSO in three different samples was compared to independent predictions of Fick's law and the modified triphasic biomechanical model by Abazari etal. (2011) with the empirical data more closely agreeing with the triphasic model.
Conclusion: Dynamic NMR spectroscopic imaging can measure spatial and temporal changes in water and cryoprotectant concentrations in articular cartilage. The modified triphasic model predictions for the interstitial distribution of DMSO were confirmed and its advantage over the predictions by Fick's law model, which is commonly used in the literature of cryobiology, was demonstrated.

• 出版日期2012-9